| Molecular Formula | C9H4O3.H2O |
| Physical and Chemical Properties | Physical and chemical properties ninhydrin is also known as "indantrione", "hydrated ninhydrin", "Ninghai Group". Commonly used developer for detection of α-amino acids and peptides. White columnar crystal turns red at 125 ℃ or left for too long, decomposes at 239~240 ℃, slightly soluble in water, easily soluble in organic solvents such as acetone and butanol. When α-amino acids are encountered in solution, or any substance containing α-amino can form dark blue or even pink or red substances, so it is often used as a prion degradation product and α-amino compound reagent. This reaction is also called "ninhydrin reaction". |
| Use | Uses: Used as an analytical reagent to identify amino acids, proteins, peptides, vitamin C, etc. It can also be used in organic synthesis. Avoid contact with skin to avoid skin redness. |
Ninhydrin
Ninhydrin is a hydrate of ninhydrin. Molecular formula C9H6O4. Molecular weight 178.14. Pale yellow columnar crystals. Melting point 241 ℃ (decomposition). Heat to above 100 ℃ and turn red. Easily soluble in water and ethanol, slightly soluble in ether and chloroform. It can be prepared by cyclization, hydrolysis, and decarboxylation of diethyl phthalate and ethyl acetate to obtain indanedione, and then oxidized and hydrated with selenium dioxide to obtain
ninhydrin staining method to identify the activity of schistosome eggs
ninhydrin staining can be used to identify the activity of schistosome eggs. Schistosoma eggs contain amber dehydrogenase. After the eggs die, this enzyme activity disappears. It can be stained with 2: 3: 5 triphenyl tetrazolium chloride and ninhydrin respectively to show the activity of eggs. Results: live eggs were orange, purple, purple, blue purple. Dead eggs are blue-gray (recently degenerated eggs) and colorless (long-term degenerated eggs).
ninhydrin reaction
Ninhydrin reaction, also known as hydrated ninhydrin reaction, is a method for testing α-amino acids. Hydrohydrated ninhydrin reacts with α-amino acids to produce blue, purple or purplish red products, but it also reacts with primary amines, other primary amino acids, ammonia, and ammonium salts. Do not react with N-substituted α, β and & gamma;-amino acids, proline, hydroxyproline. This reaction is often used for quantitative analysis of α-amino acids and color development by liquid chromatography.
If ninhydrin reacts with amino acids, it will cause oxidative deamination of amino acids. At the same time, the reduced ninhydrin reacts with the same molecule to form Rowan purple. This reaction is extremely specific as an amino acid detection method, so it can be used as a developer for paper layer chromatography or amino acid analyzer.
Ninhydrin is an organic compound. The following is an introduction to some properties, uses, preparation methods, and safety information of ninhydrin:
Nature:
-Ninhydrin is a colorless crystalline solid with a vanilla like odor.
-It is soluble in alcohols, ethers, and ketones, but insoluble in water.
-Ninhydrin is stable in air and decomposes at high temperatures.
Usage:
-Ninhydrin is often used as a dye intermediate and plays an important role in organic synthesis. It can be used to synthesize organic compounds such as pigments and fluorescent whitening agents.
Method:
-Ninhydrin can be prepared by condensation reaction of dicarbonyl compounds such as meta phenylenedione and giant vine dyes. There are various specific synthesis methods, and one commonly used method is to react 2-bromo-p-phenylenediamine with alkaline solution to obtain ninhydrin.
Security information:
-Ninhydrin is an organic compound that may have certain toxicity. When handling, avoid inhaling its dust and coming into contact with its skin.
-When using ninhydrin, appropriate protective equipment should be worn, such as gloves, goggles, and protective clothing.
-It should be stored in a dry, cool place, away from sources of fire and oxidants.
When using ninhydrin or other chemicals, please follow the relevant safety operation guidelines and carry out specific operations according to the actual situation.